Publication Date: 2010 Sep PMID: 20717154
Authors: Urnov, F. D. - Rebar, E. J. - Holmes, M. C. - Zhang, H. S. - Gregory, P. D.
Journal: Nat Rev Genet
Reverse genetics in model organisms such as Drosophila melanogaster, Arabidopsis thaliana, zebrafish and rats, efficient genome engineering in human embryonic stem and induced pluripotent stem cells, targeted integration in crop plants, and HIV resistance in immune cells - this broad range of outcomes has resulted from the application of the same core technology: targeted genome cleavage by engineered, sequence-specific zinc finger nucleases followed by gene modification during subsequent repair. Such 'genome editing' is now established in human cells and a number of model organisms, thus opening the door to a range of new experimental and therapeutic possibilities.
post to: CiteULike
Authors: Urnov, F. D. - Rebar, E. J. - Holmes, M. C. - Zhang, H. S. - Gregory, P. D.
Journal: Nat Rev Genet
Reverse genetics in model organisms such as Drosophila melanogaster, Arabidopsis thaliana, zebrafish and rats, efficient genome engineering in human embryonic stem and induced pluripotent stem cells, targeted integration in crop plants, and HIV resistance in immune cells - this broad range of outcomes has resulted from the application of the same core technology: targeted genome cleavage by engineered, sequence-specific zinc finger nucleases followed by gene modification during subsequent repair. Such 'genome editing' is now established in human cells and a number of model organisms, thus opening the door to a range of new experimental and therapeutic possibilities.
post to: CiteULike
Filed under Genetics Publications by 
Publication Date: 2010 Sep PMID: 20717155
Authors: Schadt, E. E. - Linderman, M. D. - Sorenson, J. - Lee, L. - Nolan, G. P.
Journal: Nat Rev Genet
Today we can generate hundreds of gigabases of DNA and RNA sequencing data in a week for less than US$5,000. The astonishing rate of data generation by these low-cost, high-throughput technologies in genomics is being matched by that of other technologies, such as real-time imaging and mass spectrometry-based flow cytometry. Success in the life sciences will depend on our ability to properly interpret the large-scale, high-dimensional data sets that are generated by these technologies, which in turn requires us to adopt advances in informatics. Here we discuss how we can master the different types of computational environments that exist - such as cloud and heterogeneous computing - to successfully tackle our big data problems.
post to: CiteULike
Authors: Schadt, E. E. - Linderman, M. D. - Sorenson, J. - Lee, L. - Nolan, G. P.
Journal: Nat Rev Genet
Today we can generate hundreds of gigabases of DNA and RNA sequencing data in a week for less than US$5,000. The astonishing rate of data generation by these low-cost, high-throughput technologies in genomics is being matched by that of other technologies, such as real-time imaging and mass spectrometry-based flow cytometry. Success in the life sciences will depend on our ability to properly interpret the large-scale, high-dimensional data sets that are generated by these technologies, which in turn requires us to adopt advances in informatics. Here we discuss how we can master the different types of computational environments that exist - such as cloud and heterogeneous computing - to successfully tackle our big data problems.
post to: CiteULike
Filed under Genetics Publications by
Publication Date: 2010 Sep PMID: 20717156
Authors: Coors, M. E. - Glover, J. J. - Juengst, E. T. - Sikela, J. M.
Journal: Nat Rev Genet
A flood of comparative genomic data is resulting in the identification of human lineage-specific (HLS) sequences. As apes are our closest evolutionary relatives, transgenic introduction of HLS sequences into these species has the greatest potential to produce 'humanized' phenotypes and also to illuminate the functions of these sequences. We argue that such transgenic apes would also be more likely than other species to experience harm from such research, which renders such studies ethically unacceptable in apes and justifies regulatory barriers between these species and other non-human primates for HLS transgenic research.
post to: CiteULike
Authors: Coors, M. E. - Glover, J. J. - Juengst, E. T. - Sikela, J. M.
Journal: Nat Rev Genet
A flood of comparative genomic data is resulting in the identification of human lineage-specific (HLS) sequences. As apes are our closest evolutionary relatives, transgenic introduction of HLS sequences into these species has the greatest potential to produce 'humanized' phenotypes and also to illuminate the functions of these sequences. We argue that such transgenic apes would also be more likely than other species to experience harm from such research, which renders such studies ethically unacceptable in apes and justifies regulatory barriers between these species and other non-human primates for HLS transgenic research.
post to: CiteULike
Filed under Genetics Publications by
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